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A and C , representative heatmaps of chromosome 2 <t>showing</t> <t>Hi-C</t> correlation matrices at 250-kb resolution for H4 cells (A) and MOG-G-UVW cells (C) infected with adeno-STAG2 or vector alone. Principal Component 1 (PC1) values define the A (green) or B (red) compartment status of individual 250-kb bins. B and D , scatterplot analysis depicting the effect of rapid STAG2 reconstitution on compartment assignments in H4 cells (B) and MOG-G-UVW cells (D). Bins undergoing compartment switches (p<0.01) are indicated in red (B to A) and blue (A to B). Coefficient of determination ( R ²) and the total number of bins (n) are indicated.
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A and C , representative heatmaps of chromosome 2 showing Hi-C correlation matrices at 250-kb resolution for H4 cells (A) and MOG-G-UVW cells (C) infected with adeno-STAG2 or vector alone. Principal Component 1 (PC1) values define the A (green) or B (red) compartment status of individual 250-kb bins. B and D , scatterplot analysis depicting the effect of rapid STAG2 reconstitution on compartment assignments in H4 cells (B) and MOG-G-UVW cells (D). Bins undergoing compartment switches (p<0.01) are indicated in red (B to A) and blue (A to B). Coefficient of determination ( R ²) and the total number of bins (n) are indicated.

Journal: bioRxiv

Article Title: Rapid reduction in global chromatin loop size after acute STAG2 reconstitution in human cancer cells

doi: 10.64898/2026.05.26.727913

Figure Lengend Snippet: A and C , representative heatmaps of chromosome 2 showing Hi-C correlation matrices at 250-kb resolution for H4 cells (A) and MOG-G-UVW cells (C) infected with adeno-STAG2 or vector alone. Principal Component 1 (PC1) values define the A (green) or B (red) compartment status of individual 250-kb bins. B and D , scatterplot analysis depicting the effect of rapid STAG2 reconstitution on compartment assignments in H4 cells (B) and MOG-G-UVW cells (D). Bins undergoing compartment switches (p<0.01) are indicated in red (B to A) and blue (A to B). Coefficient of determination ( R ²) and the total number of bins (n) are indicated.

Article Snippet: Hi-C libraries were generated using the Arima Hi-C+ kit (Arima Genomics) according to the manufacturer’s instructions.

Techniques: Hi-C, Infection, Plasmid Preparation